用于浸出铀矿的细菌选育方法及所选育细菌的应用

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用于浸出铀矿的细菌选育方法及所选育细菌的应用

本发明涉及一种用于浸出铀矿的细菌选育方法及所选育细菌的应用。其分两个阶段进行细菌选育：第一阶段，向培养基中加入铀矿石配成一定浓度的矿浆，然后接种入氧化亚铁硫杆菌，进行驯化选育细菌；第二阶段，向培养基中加入有害离子配成一定浓度的含有害离子的溶液，然后接种入第一阶段驯化选育所得细菌，进行驯化选育，得到最终所选育细菌。通过本发明方法选育得到的细菌活性高，生长繁殖过程中诱导期、缓慢期会大大缩短或消失。本发明应用于浸出铀矿，铀浸出速度明显提高，酸耗降低，浸出液体积大大减少，为后续工艺降低试剂消耗、能耗创造了便利条件。

Selective breeding method ofbacteria used for leaching uranium ore and application of the selectively bredbacteria

The invention relates to aselective breeding method of bacteria used for leaching uranium ore and anapplication of the selectively bred bacteria. The method includes two steps forthe selective breeding: step 1), adding uranium ore to a culture medium toprepare ore pulp in certain concentration and inoculating thiobacillusferrooxidans to perform domestication and selective breeding on bacteria; step2), adding harmful ions to the culture medium to prepare a solution containing theharmful ions in certain concentration and inoculating the bacteria obtained instep 1) through domestication and selective breeding to perform domesticationand selective breeding, thereby obtaining the final selectively bred bacteria.According to the method, the selectively bred bacteria are high in activity,wherein an induction period and a slow period during growth and reproductionare greatly reduced or even eliminated. The bacteria are used for leaching theuranium ore, are significantly increased in leaching speed of the uranium, arereduced in acid consumption, are greatly reduced in volume of a leachate, andbring convenience to reduction on reagent consumption and energy consumption insubsequent processes.